The role of palmitoylation for protein recruitment to the inner membrane complex of the malaria parasite.
Identifieur interne : 000491 ( Main/Exploration ); précédent : 000490; suivant : 000492The role of palmitoylation for protein recruitment to the inner membrane complex of the malaria parasite.
Auteurs : Johanna Wetzel [Canada] ; Susann Herrmann [Canada] ; Lakshmipuram Seshadri Swapna [Canada] ; Dhaneswar Prusty [Canada] ; Arun T. John Peter [Allemagne] ; Maya Kono ; Sidharth Saini [Canada] ; Srinivas Nellimarla [Canada] ; Tatianna Wai Ying Wong [Canada] ; Louisa Wilcke [Canada] ; Olivia Ramsay [Canada] ; Ana Cabrera [Canada] ; Laura Biller ; Dorothee Heincke [Canada] ; Karen Mossman [Canada] ; Tobias Spielmann ; Christian Ungermann [Allemagne] ; John Parkinson [Canada] ; Tim W. Gilberger [Canada]Source :
- The Journal of biological chemistry [ 1083-351X ] ; 2015.
English descriptors
- KwdEn :
- Actins (chemistry), Acyltransferases (metabolism), Biotin (chemistry), Catalysis, Cell Membrane (metabolism), DNA Mutational Analysis, Green Fluorescent Proteins (metabolism), Humans, Malaria (parasitology), Membrane Proteins (metabolism), Phylogeny, Plasmodium falciparum (metabolism), Protein Structure, Tertiary, Protein Transport, Protozoan Proteins (metabolism).
- MESH :
- chemical , chemistry : Actins, Biotin.
- chemical , metabolism : Acyltransferases, Green Fluorescent Proteins, Membrane Proteins, Protozoan Proteins.
- metabolism : Cell Membrane, Plasmodium falciparum.
- parasitology : Malaria.
- Catalysis, DNA Mutational Analysis, Humans, Phylogeny, Protein Structure, Tertiary, Protein Transport.
Abstract
To survive and persist within its human host, the malaria parasite Plasmodium falciparum utilizes a battery of lineage-specific innovations to invade and multiply in human erythrocytes. With central roles in invasion and cytokinesis, the inner membrane complex, a Golgi-derived double membrane structure underlying the plasma membrane of the parasite, represents a unique and unifying structure characteristic to all organisms belonging to a large phylogenetic group called Alveolata. More than 30 structurally and phylogenetically distinct proteins are embedded in the IMC, where a portion of these proteins displays N-terminal acylation motifs. Although N-terminal myristoylation is catalyzed co-translationally within the cytoplasm of the parasite, palmitoylation takes place at membranes and is mediated by palmitoyl acyltransferases (PATs). Here, we identify a PAT (PfDHHC1) that is exclusively localized to the IMC. Systematic phylogenetic analysis of the alveolate PAT family reveals PfDHHC1 to be a member of a highly conserved, apicomplexan-specific clade of PATs. We show that during schizogony this enzyme has an identical distribution like two dual-acylated, IMC-localized proteins (PfISP1 and PfISP3). We used these proteins to probe into specific sequence requirements for IMC-specific membrane recruitment and their interaction with differentially localized PATs of the parasite.
DOI: 10.1074/jbc.M114.598094
PubMed: 25425642
Affiliations:
- Allemagne, Canada
- Basse-Saxe, Ontario
- Hamilton (Ontario), Osnabrück, Toronto
- Université McMaster, Université de Toronto
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Le document en format XML
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DeGroote Institute for Infectious Disease Research, Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario L8N 3Z5</wicri:regionArea><orgName type="university">Université McMaster</orgName><placeName><settlement type="city">Hamilton (Ontario)</settlement><region type="state">Ontario</region></placeName></affiliation></author><author><name sortKey="Herrmann, Susann" sort="Herrmann, Susann" uniqKey="Herrmann S" first="Susann" last="Herrmann">Susann Herrmann</name><affiliation wicri:level="4"><nlm:affiliation>From the M. G. DeGroote Institute for Infectious Disease Research, Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario L8N 3Z5, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>From the M. G. DeGroote Institute for Infectious Disease Research, Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario L8N 3Z5</wicri:regionArea><orgName type="university">Université McMaster</orgName><placeName><settlement type="city">Hamilton (Ontario)</settlement><region type="state">Ontario</region></placeName></affiliation></author><author><name sortKey="Swapna, Lakshmipuram Seshadri" sort="Swapna, Lakshmipuram Seshadri" uniqKey="Swapna L" first="Lakshmipuram Seshadri" last="Swapna">Lakshmipuram Seshadri Swapna</name><affiliation wicri:level="4"><nlm:affiliation>the Program in Molecular Structure and Function, Hospital for Sick Children, and Departments of Biochemistry and Molecular Genetics, University of Toronto, Toronto, Ontario M5G 0A4, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>the Program in Molecular Structure and Function, Hospital for Sick Children, and Departments of Biochemistry and Molecular Genetics, University of Toronto, Toronto, Ontario M5G 0A4</wicri:regionArea><orgName type="university">Université de Toronto</orgName><placeName><settlement type="city">Toronto</settlement><region type="state">Ontario</region></placeName></affiliation></author><author><name sortKey="Prusty, Dhaneswar" sort="Prusty, Dhaneswar" uniqKey="Prusty D" first="Dhaneswar" last="Prusty">Dhaneswar Prusty</name><affiliation wicri:level="4"><nlm:affiliation>From the M. G. DeGroote Institute for Infectious Disease Research, Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario L8N 3Z5, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>From the M. G. DeGroote Institute for Infectious Disease Research, Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario L8N 3Z5</wicri:regionArea><orgName type="university">Université McMaster</orgName><placeName><settlement type="city">Hamilton (Ontario)</settlement><region type="state">Ontario</region></placeName></affiliation></author><author><name sortKey="John Peter, Arun T" sort="John Peter, Arun T" uniqKey="John Peter A" first="Arun T" last="John Peter">Arun T. John Peter</name><affiliation wicri:level="3"><nlm:affiliation>the Department of Biology/Chemistry, Biochemistry Section, University of Osnabrück, 49076 Osnabrück, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>the Department of Biology/Chemistry, Biochemistry Section, University of Osnabrück, 49076 Osnabrück</wicri:regionArea><placeName><region type="land" nuts="2">Basse-Saxe</region><settlement type="city">Osnabrück</settlement></placeName></affiliation></author><author><name sortKey="Kono, Maya" sort="Kono, Maya" uniqKey="Kono M" first="Maya" last="Kono">Maya Kono</name><affiliation><nlm:affiliation>the Bernhard-Nocht-Institute for Tropical Medicine, 20359 Hamburg, Germany, and.</nlm:affiliation><wicri:noCountry code="subField">and</wicri:noCountry></affiliation></author><author><name sortKey="Saini, Sidharth" sort="Saini, Sidharth" uniqKey="Saini S" first="Sidharth" last="Saini">Sidharth Saini</name><affiliation wicri:level="4"><nlm:affiliation>From the M. G. DeGroote Institute for Infectious Disease Research, Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario L8N 3Z5, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>From the M. G. DeGroote Institute for Infectious Disease Research, Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario L8N 3Z5</wicri:regionArea><orgName type="university">Université McMaster</orgName><placeName><settlement type="city">Hamilton (Ontario)</settlement><region type="state">Ontario</region></placeName></affiliation></author><author><name sortKey="Nellimarla, Srinivas" sort="Nellimarla, Srinivas" uniqKey="Nellimarla S" first="Srinivas" last="Nellimarla">Srinivas Nellimarla</name><affiliation wicri:level="4"><nlm:affiliation>From the M. G. DeGroote Institute for Infectious Disease Research, Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario L8N 3Z5, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>From the M. G. DeGroote Institute for Infectious Disease Research, Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario L8N 3Z5</wicri:regionArea><orgName type="university">Université McMaster</orgName><placeName><settlement type="city">Hamilton (Ontario)</settlement><region type="state">Ontario</region></placeName></affiliation></author><author><name sortKey="Wong, Tatianna Wai Ying" sort="Wong, Tatianna Wai Ying" uniqKey="Wong T" first="Tatianna Wai Ying" last="Wong">Tatianna Wai Ying Wong</name><affiliation wicri:level="4"><nlm:affiliation>From the M. G. DeGroote Institute for Infectious Disease Research, Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario L8N 3Z5, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>From the M. G. DeGroote Institute for Infectious Disease Research, Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario L8N 3Z5</wicri:regionArea><orgName type="university">Université McMaster</orgName><placeName><settlement type="city">Hamilton (Ontario)</settlement><region type="state">Ontario</region></placeName></affiliation></author><author><name sortKey="Wilcke, Louisa" sort="Wilcke, Louisa" uniqKey="Wilcke L" first="Louisa" last="Wilcke">Louisa Wilcke</name><affiliation wicri:level="4"><nlm:affiliation>From the M. G. DeGroote Institute for Infectious Disease Research, Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario L8N 3Z5, Canada, the Bernhard-Nocht-Institute for Tropical Medicine, 20359 Hamburg, Germany, and.</nlm:affiliation><orgName type="university">Université McMaster</orgName><country>Canada</country><placeName><settlement type="city">Hamilton (Ontario)</settlement><region type="state">Ontario</region></placeName></affiliation></author><author><name sortKey="Ramsay, Olivia" sort="Ramsay, Olivia" uniqKey="Ramsay O" first="Olivia" last="Ramsay">Olivia Ramsay</name><affiliation wicri:level="4"><nlm:affiliation>From the M. G. DeGroote Institute for Infectious Disease Research, Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario L8N 3Z5, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>From the M. G. DeGroote Institute for Infectious Disease Research, Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario L8N 3Z5</wicri:regionArea><orgName type="university">Université McMaster</orgName><placeName><settlement type="city">Hamilton (Ontario)</settlement><region type="state">Ontario</region></placeName></affiliation></author><author><name sortKey="Cabrera, Ana" sort="Cabrera, Ana" uniqKey="Cabrera A" first="Ana" last="Cabrera">Ana Cabrera</name><affiliation wicri:level="4"><nlm:affiliation>From the M. G. DeGroote Institute for Infectious Disease Research, Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario L8N 3Z5, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>From the M. G. DeGroote Institute for Infectious Disease Research, Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario L8N 3Z5</wicri:regionArea><orgName type="university">Université McMaster</orgName><placeName><settlement type="city">Hamilton (Ontario)</settlement><region type="state">Ontario</region></placeName></affiliation></author><author><name sortKey="Biller, Laura" sort="Biller, Laura" uniqKey="Biller L" first="Laura" last="Biller">Laura Biller</name><affiliation><nlm:affiliation>the Bernhard-Nocht-Institute for Tropical Medicine, 20359 Hamburg, Germany, and.</nlm:affiliation><wicri:noCountry code="subField">and</wicri:noCountry></affiliation></author><author><name sortKey="Heincke, Dorothee" sort="Heincke, Dorothee" uniqKey="Heincke D" first="Dorothee" last="Heincke">Dorothee Heincke</name><affiliation wicri:level="4"><nlm:affiliation>From the M. G. DeGroote Institute for Infectious Disease Research, Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario L8N 3Z5, Canada, the Bernhard-Nocht-Institute for Tropical Medicine, 20359 Hamburg, Germany, and.</nlm:affiliation><orgName type="university">Université McMaster</orgName><country>Canada</country><placeName><settlement type="city">Hamilton (Ontario)</settlement><region type="state">Ontario</region></placeName></affiliation></author><author><name sortKey="Mossman, Karen" sort="Mossman, Karen" uniqKey="Mossman K" first="Karen" last="Mossman">Karen Mossman</name><affiliation wicri:level="4"><nlm:affiliation>From the M. G. DeGroote Institute for Infectious Disease Research, Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario L8N 3Z5, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>From the M. G. DeGroote Institute for Infectious Disease Research, Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario L8N 3Z5</wicri:regionArea><orgName type="university">Université McMaster</orgName><placeName><settlement type="city">Hamilton (Ontario)</settlement><region type="state">Ontario</region></placeName></affiliation></author><author><name sortKey="Spielmann, Tobias" sort="Spielmann, Tobias" uniqKey="Spielmann T" first="Tobias" last="Spielmann">Tobias Spielmann</name><affiliation><nlm:affiliation>the Bernhard-Nocht-Institute for Tropical Medicine, 20359 Hamburg, Germany, and.</nlm:affiliation><wicri:noCountry code="subField">and</wicri:noCountry></affiliation></author><author><name sortKey="Ungermann, Christian" sort="Ungermann, Christian" uniqKey="Ungermann C" first="Christian" last="Ungermann">Christian Ungermann</name><affiliation wicri:level="3"><nlm:affiliation>the Department of Biology/Chemistry, Biochemistry Section, University of Osnabrück, 49076 Osnabrück, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>the Department of Biology/Chemistry, Biochemistry Section, University of Osnabrück, 49076 Osnabrück</wicri:regionArea><placeName><region type="land" nuts="2">Basse-Saxe</region><settlement type="city">Osnabrück</settlement></placeName></affiliation></author><author><name sortKey="Parkinson, John" sort="Parkinson, John" uniqKey="Parkinson J" first="John" last="Parkinson">John Parkinson</name><affiliation wicri:level="4"><nlm:affiliation>the Program in Molecular Structure and Function, Hospital for Sick Children, and Departments of Biochemistry and Molecular Genetics, University of Toronto, Toronto, Ontario M5G 0A4, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>the Program in Molecular Structure and Function, Hospital for Sick Children, and Departments of Biochemistry and Molecular Genetics, University of Toronto, Toronto, Ontario M5G 0A4</wicri:regionArea><orgName type="university">Université de Toronto</orgName><placeName><settlement type="city">Toronto</settlement><region type="state">Ontario</region></placeName></affiliation></author><author><name sortKey="Gilberger, Tim W" sort="Gilberger, Tim W" uniqKey="Gilberger T" first="Tim W" last="Gilberger">Tim W. Gilberger</name><affiliation wicri:level="4"><nlm:affiliation>From the M. G. DeGroote Institute for Infectious Disease Research, Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario L8N 3Z5, Canada, the Bernhard-Nocht-Institute for Tropical Medicine, 20359 Hamburg, Germany, and the Center for Structural Systems Biology, 22607 Hamburg, Germany tgilber@mcmaster.ca.</nlm:affiliation><country wicri:rule="url">Canada</country><wicri:regionArea>From the M. G. DeGroote Institute for Infectious Disease Research, Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario L8N 3Z5, Canada, the Bernhard-Nocht-Institute for Tropical Medicine, 20359 Hamburg, Germany, and the Center for Structural Systems Biology, 22607 Hamburg</wicri:regionArea><orgName type="university">Université McMaster</orgName><placeName><settlement type="city">Hamilton (Ontario)</settlement><region type="state">Ontario</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2015">2015</date><idno type="RBID">pubmed:25425642</idno><idno type="pmid">25425642</idno><idno type="doi">10.1074/jbc.M114.598094</idno><idno type="wicri:Area/PubMed/Corpus">000585</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000585</idno><idno type="wicri:Area/PubMed/Curation">000585</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000585</idno><idno type="wicri:Area/PubMed/Checkpoint">000585</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000585</idno><idno type="wicri:Area/Ncbi/Merge">001973</idno><idno type="wicri:Area/Ncbi/Curation">001973</idno><idno type="wicri:Area/Ncbi/Checkpoint">001973</idno><idno type="wicri:Area/Main/Merge">000492</idno><idno type="wicri:Area/Main/Curation">000491</idno><idno type="wicri:Area/Main/Exploration">000491</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">The role of palmitoylation for protein recruitment to the inner membrane complex of the malaria parasite.</title><author><name sortKey="Wetzel, Johanna" sort="Wetzel, Johanna" uniqKey="Wetzel J" first="Johanna" last="Wetzel">Johanna Wetzel</name><affiliation wicri:level="4"><nlm:affiliation>From the M. G. DeGroote Institute for Infectious Disease Research, Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario L8N 3Z5, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>From the M. G. DeGroote Institute for Infectious Disease Research, Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario L8N 3Z5</wicri:regionArea><orgName type="university">Université McMaster</orgName><placeName><settlement type="city">Hamilton (Ontario)</settlement><region type="state">Ontario</region></placeName></affiliation></author><author><name sortKey="Herrmann, Susann" sort="Herrmann, Susann" uniqKey="Herrmann S" first="Susann" last="Herrmann">Susann Herrmann</name><affiliation wicri:level="4"><nlm:affiliation>From the M. G. DeGroote Institute for Infectious Disease Research, Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario L8N 3Z5, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>From the M. G. DeGroote Institute for Infectious Disease Research, Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario L8N 3Z5</wicri:regionArea><orgName type="university">Université McMaster</orgName><placeName><settlement type="city">Hamilton (Ontario)</settlement><region type="state">Ontario</region></placeName></affiliation></author><author><name sortKey="Swapna, Lakshmipuram Seshadri" sort="Swapna, Lakshmipuram Seshadri" uniqKey="Swapna L" first="Lakshmipuram Seshadri" last="Swapna">Lakshmipuram Seshadri Swapna</name><affiliation wicri:level="4"><nlm:affiliation>the Program in Molecular Structure and Function, Hospital for Sick Children, and Departments of Biochemistry and Molecular Genetics, University of Toronto, Toronto, Ontario M5G 0A4, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>the Program in Molecular Structure and Function, Hospital for Sick Children, and Departments of Biochemistry and Molecular Genetics, University of Toronto, Toronto, Ontario M5G 0A4</wicri:regionArea><orgName type="university">Université de Toronto</orgName><placeName><settlement type="city">Toronto</settlement><region type="state">Ontario</region></placeName></affiliation></author><author><name sortKey="Prusty, Dhaneswar" sort="Prusty, Dhaneswar" uniqKey="Prusty D" first="Dhaneswar" last="Prusty">Dhaneswar Prusty</name><affiliation wicri:level="4"><nlm:affiliation>From the M. G. DeGroote Institute for Infectious Disease Research, Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario L8N 3Z5, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>From the M. G. DeGroote Institute for Infectious Disease Research, Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario L8N 3Z5</wicri:regionArea><orgName type="university">Université McMaster</orgName><placeName><settlement type="city">Hamilton (Ontario)</settlement><region type="state">Ontario</region></placeName></affiliation></author><author><name sortKey="John Peter, Arun T" sort="John Peter, Arun T" uniqKey="John Peter A" first="Arun T" last="John Peter">Arun T. John Peter</name><affiliation wicri:level="3"><nlm:affiliation>the Department of Biology/Chemistry, Biochemistry Section, University of Osnabrück, 49076 Osnabrück, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>the Department of Biology/Chemistry, Biochemistry Section, University of Osnabrück, 49076 Osnabrück</wicri:regionArea><placeName><region type="land" nuts="2">Basse-Saxe</region><settlement type="city">Osnabrück</settlement></placeName></affiliation></author><author><name sortKey="Kono, Maya" sort="Kono, Maya" uniqKey="Kono M" first="Maya" last="Kono">Maya Kono</name><affiliation><nlm:affiliation>the Bernhard-Nocht-Institute for Tropical Medicine, 20359 Hamburg, Germany, and.</nlm:affiliation><wicri:noCountry code="subField">and</wicri:noCountry></affiliation></author><author><name sortKey="Saini, Sidharth" sort="Saini, Sidharth" uniqKey="Saini S" first="Sidharth" last="Saini">Sidharth Saini</name><affiliation wicri:level="4"><nlm:affiliation>From the M. G. DeGroote Institute for Infectious Disease Research, Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario L8N 3Z5, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>From the M. G. DeGroote Institute for Infectious Disease Research, Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario L8N 3Z5</wicri:regionArea><orgName type="university">Université McMaster</orgName><placeName><settlement type="city">Hamilton (Ontario)</settlement><region type="state">Ontario</region></placeName></affiliation></author><author><name sortKey="Nellimarla, Srinivas" sort="Nellimarla, Srinivas" uniqKey="Nellimarla S" first="Srinivas" last="Nellimarla">Srinivas Nellimarla</name><affiliation wicri:level="4"><nlm:affiliation>From the M. G. DeGroote Institute for Infectious Disease Research, Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario L8N 3Z5, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>From the M. G. DeGroote Institute for Infectious Disease Research, Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario L8N 3Z5</wicri:regionArea><orgName type="university">Université McMaster</orgName><placeName><settlement type="city">Hamilton (Ontario)</settlement><region type="state">Ontario</region></placeName></affiliation></author><author><name sortKey="Wong, Tatianna Wai Ying" sort="Wong, Tatianna Wai Ying" uniqKey="Wong T" first="Tatianna Wai Ying" last="Wong">Tatianna Wai Ying Wong</name><affiliation wicri:level="4"><nlm:affiliation>From the M. G. DeGroote Institute for Infectious Disease Research, Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario L8N 3Z5, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>From the M. G. DeGroote Institute for Infectious Disease Research, Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario L8N 3Z5</wicri:regionArea><orgName type="university">Université McMaster</orgName><placeName><settlement type="city">Hamilton (Ontario)</settlement><region type="state">Ontario</region></placeName></affiliation></author><author><name sortKey="Wilcke, Louisa" sort="Wilcke, Louisa" uniqKey="Wilcke L" first="Louisa" last="Wilcke">Louisa Wilcke</name><affiliation wicri:level="4"><nlm:affiliation>From the M. G. DeGroote Institute for Infectious Disease Research, Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario L8N 3Z5, Canada, the Bernhard-Nocht-Institute for Tropical Medicine, 20359 Hamburg, Germany, and.</nlm:affiliation><orgName type="university">Université McMaster</orgName><country>Canada</country><placeName><settlement type="city">Hamilton (Ontario)</settlement><region type="state">Ontario</region></placeName></affiliation></author><author><name sortKey="Ramsay, Olivia" sort="Ramsay, Olivia" uniqKey="Ramsay O" first="Olivia" last="Ramsay">Olivia Ramsay</name><affiliation wicri:level="4"><nlm:affiliation>From the M. G. DeGroote Institute for Infectious Disease Research, Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario L8N 3Z5, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>From the M. G. DeGroote Institute for Infectious Disease Research, Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario L8N 3Z5</wicri:regionArea><orgName type="university">Université McMaster</orgName><placeName><settlement type="city">Hamilton (Ontario)</settlement><region type="state">Ontario</region></placeName></affiliation></author><author><name sortKey="Cabrera, Ana" sort="Cabrera, Ana" uniqKey="Cabrera A" first="Ana" last="Cabrera">Ana Cabrera</name><affiliation wicri:level="4"><nlm:affiliation>From the M. G. DeGroote Institute for Infectious Disease Research, Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario L8N 3Z5, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>From the M. G. DeGroote Institute for Infectious Disease Research, Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario L8N 3Z5</wicri:regionArea><orgName type="university">Université McMaster</orgName><placeName><settlement type="city">Hamilton (Ontario)</settlement><region type="state">Ontario</region></placeName></affiliation></author><author><name sortKey="Biller, Laura" sort="Biller, Laura" uniqKey="Biller L" first="Laura" last="Biller">Laura Biller</name><affiliation><nlm:affiliation>the Bernhard-Nocht-Institute for Tropical Medicine, 20359 Hamburg, Germany, and.</nlm:affiliation><wicri:noCountry code="subField">and</wicri:noCountry></affiliation></author><author><name sortKey="Heincke, Dorothee" sort="Heincke, Dorothee" uniqKey="Heincke D" first="Dorothee" last="Heincke">Dorothee Heincke</name><affiliation wicri:level="4"><nlm:affiliation>From the M. G. DeGroote Institute for Infectious Disease Research, Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario L8N 3Z5, Canada, the Bernhard-Nocht-Institute for Tropical Medicine, 20359 Hamburg, Germany, and.</nlm:affiliation><orgName type="university">Université McMaster</orgName><country>Canada</country><placeName><settlement type="city">Hamilton (Ontario)</settlement><region type="state">Ontario</region></placeName></affiliation></author><author><name sortKey="Mossman, Karen" sort="Mossman, Karen" uniqKey="Mossman K" first="Karen" last="Mossman">Karen Mossman</name><affiliation wicri:level="4"><nlm:affiliation>From the M. G. DeGroote Institute for Infectious Disease Research, Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario L8N 3Z5, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>From the M. G. DeGroote Institute for Infectious Disease Research, Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario L8N 3Z5</wicri:regionArea><orgName type="university">Université McMaster</orgName><placeName><settlement type="city">Hamilton (Ontario)</settlement><region type="state">Ontario</region></placeName></affiliation></author><author><name sortKey="Spielmann, Tobias" sort="Spielmann, Tobias" uniqKey="Spielmann T" first="Tobias" last="Spielmann">Tobias Spielmann</name><affiliation><nlm:affiliation>the Bernhard-Nocht-Institute for Tropical Medicine, 20359 Hamburg, Germany, and.</nlm:affiliation><wicri:noCountry code="subField">and</wicri:noCountry></affiliation></author><author><name sortKey="Ungermann, Christian" sort="Ungermann, Christian" uniqKey="Ungermann C" first="Christian" last="Ungermann">Christian Ungermann</name><affiliation wicri:level="3"><nlm:affiliation>the Department of Biology/Chemistry, Biochemistry Section, University of Osnabrück, 49076 Osnabrück, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>the Department of Biology/Chemistry, Biochemistry Section, University of Osnabrück, 49076 Osnabrück</wicri:regionArea><placeName><region type="land" nuts="2">Basse-Saxe</region><settlement type="city">Osnabrück</settlement></placeName></affiliation></author><author><name sortKey="Parkinson, John" sort="Parkinson, John" uniqKey="Parkinson J" first="John" last="Parkinson">John Parkinson</name><affiliation wicri:level="4"><nlm:affiliation>the Program in Molecular Structure and Function, Hospital for Sick Children, and Departments of Biochemistry and Molecular Genetics, University of Toronto, Toronto, Ontario M5G 0A4, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>the Program in Molecular Structure and Function, Hospital for Sick Children, and Departments of Biochemistry and Molecular Genetics, University of Toronto, Toronto, Ontario M5G 0A4</wicri:regionArea><orgName type="university">Université de Toronto</orgName><placeName><settlement type="city">Toronto</settlement><region type="state">Ontario</region></placeName></affiliation></author><author><name sortKey="Gilberger, Tim W" sort="Gilberger, Tim W" uniqKey="Gilberger T" first="Tim W" last="Gilberger">Tim W. Gilberger</name><affiliation wicri:level="4"><nlm:affiliation>From the M. G. DeGroote Institute for Infectious Disease Research, Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario L8N 3Z5, Canada, the Bernhard-Nocht-Institute for Tropical Medicine, 20359 Hamburg, Germany, and the Center for Structural Systems Biology, 22607 Hamburg, Germany tgilber@mcmaster.ca.</nlm:affiliation><country wicri:rule="url">Canada</country><wicri:regionArea>From the M. G. DeGroote Institute for Infectious Disease Research, Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario L8N 3Z5, Canada, the Bernhard-Nocht-Institute for Tropical Medicine, 20359 Hamburg, Germany, and the Center for Structural Systems Biology, 22607 Hamburg</wicri:regionArea><orgName type="university">Université McMaster</orgName><placeName><settlement type="city">Hamilton (Ontario)</settlement><region type="state">Ontario</region></placeName></affiliation></author></analytic><series><title level="j">The Journal of biological chemistry</title><idno type="eISSN">1083-351X</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Actins (chemistry)</term><term>Acyltransferases (metabolism)</term><term>Biotin (chemistry)</term><term>Catalysis</term><term>Cell Membrane (metabolism)</term><term>DNA Mutational Analysis</term><term>Green Fluorescent Proteins (metabolism)</term><term>Humans</term><term>Malaria (parasitology)</term><term>Membrane Proteins (metabolism)</term><term>Phylogeny</term><term>Plasmodium falciparum (metabolism)</term><term>Protein Structure, Tertiary</term><term>Protein Transport</term><term>Protozoan Proteins (metabolism)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Actins</term><term>Biotin</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Acyltransferases</term><term>Green Fluorescent Proteins</term><term>Membrane Proteins</term><term>Protozoan Proteins</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cell Membrane</term><term>Plasmodium falciparum</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Malaria</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Catalysis</term><term>DNA Mutational Analysis</term><term>Humans</term><term>Phylogeny</term><term>Protein Structure, Tertiary</term><term>Protein Transport</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">To survive and persist within its human host, the malaria parasite Plasmodium falciparum utilizes a battery of lineage-specific innovations to invade and multiply in human erythrocytes. With central roles in invasion and cytokinesis, the inner membrane complex, a Golgi-derived double membrane structure underlying the plasma membrane of the parasite, represents a unique and unifying structure characteristic to all organisms belonging to a large phylogenetic group called Alveolata. More than 30 structurally and phylogenetically distinct proteins are embedded in the IMC, where a portion of these proteins displays N-terminal acylation motifs. Although N-terminal myristoylation is catalyzed co-translationally within the cytoplasm of the parasite, palmitoylation takes place at membranes and is mediated by palmitoyl acyltransferases (PATs). Here, we identify a PAT (PfDHHC1) that is exclusively localized to the IMC. Systematic phylogenetic analysis of the alveolate PAT family reveals PfDHHC1 to be a member of a highly conserved, apicomplexan-specific clade of PATs. We show that during schizogony this enzyme has an identical distribution like two dual-acylated, IMC-localized proteins (PfISP1 and PfISP3). We used these proteins to probe into specific sequence requirements for IMC-specific membrane recruitment and their interaction with differentially localized PATs of the parasite.</div></front></TEI><affiliations><list><country><li>Allemagne</li><li>Canada</li></country><region><li>Basse-Saxe</li><li>Ontario</li></region><settlement><li>Hamilton (Ontario)</li><li>Osnabrück</li><li>Toronto</li></settlement><orgName><li>Université McMaster</li><li>Université de Toronto</li></orgName></list><tree><noCountry><name sortKey="Biller, Laura" sort="Biller, Laura" uniqKey="Biller L" first="Laura" last="Biller">Laura Biller</name><name sortKey="Kono, Maya" sort="Kono, Maya" uniqKey="Kono M" first="Maya" last="Kono">Maya Kono</name><name sortKey="Spielmann, Tobias" sort="Spielmann, Tobias" uniqKey="Spielmann T" first="Tobias" last="Spielmann">Tobias Spielmann</name></noCountry><country name="Canada"><region name="Ontario"><name sortKey="Wetzel, Johanna" sort="Wetzel, Johanna" uniqKey="Wetzel J" first="Johanna" last="Wetzel">Johanna Wetzel</name></region><name sortKey="Cabrera, Ana" sort="Cabrera, Ana" uniqKey="Cabrera A" first="Ana" last="Cabrera">Ana Cabrera</name><name sortKey="Gilberger, Tim W" sort="Gilberger, Tim W" uniqKey="Gilberger T" first="Tim W" last="Gilberger">Tim W. Gilberger</name><name sortKey="Heincke, Dorothee" sort="Heincke, Dorothee" uniqKey="Heincke D" first="Dorothee" last="Heincke">Dorothee Heincke</name><name sortKey="Herrmann, Susann" sort="Herrmann, Susann" uniqKey="Herrmann S" first="Susann" last="Herrmann">Susann Herrmann</name><name sortKey="Mossman, Karen" sort="Mossman, Karen" uniqKey="Mossman K" first="Karen" last="Mossman">Karen Mossman</name><name sortKey="Nellimarla, Srinivas" sort="Nellimarla, Srinivas" uniqKey="Nellimarla S" first="Srinivas" last="Nellimarla">Srinivas Nellimarla</name><name sortKey="Parkinson, John" sort="Parkinson, John" uniqKey="Parkinson J" first="John" last="Parkinson">John Parkinson</name><name sortKey="Prusty, Dhaneswar" sort="Prusty, Dhaneswar" uniqKey="Prusty D" first="Dhaneswar" last="Prusty">Dhaneswar Prusty</name><name sortKey="Ramsay, Olivia" sort="Ramsay, Olivia" uniqKey="Ramsay O" first="Olivia" last="Ramsay">Olivia Ramsay</name><name sortKey="Saini, Sidharth" sort="Saini, Sidharth" uniqKey="Saini S" first="Sidharth" last="Saini">Sidharth Saini</name><name sortKey="Swapna, Lakshmipuram Seshadri" sort="Swapna, Lakshmipuram Seshadri" uniqKey="Swapna L" first="Lakshmipuram Seshadri" last="Swapna">Lakshmipuram Seshadri Swapna</name><name sortKey="Wilcke, Louisa" sort="Wilcke, Louisa" uniqKey="Wilcke L" first="Louisa" last="Wilcke">Louisa Wilcke</name><name sortKey="Wong, Tatianna Wai Ying" sort="Wong, Tatianna Wai Ying" uniqKey="Wong T" first="Tatianna Wai Ying" last="Wong">Tatianna Wai Ying Wong</name></country><country name="Allemagne"><region name="Basse-Saxe"><name sortKey="John Peter, Arun T" sort="John Peter, Arun T" uniqKey="John Peter A" first="Arun T" last="John Peter">Arun T. John Peter</name></region><name sortKey="Ungermann, Christian" sort="Ungermann, Christian" uniqKey="Ungermann C" first="Christian" last="Ungermann">Christian Ungermann</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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